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(1) Background: Hemoparasites of the genus Haemogregarina (Haemogregarinidae) are commonly reported in freshwater turtles. However, in Brazil, only three species have currently been characterised using molecular methods.

This study aimed to bring new insights on the diversity of species of Haemogregarina infecting Brazilian freshwater turtles from Mato Grosso and Goiás states using molecular and morphological tools. (2) Methods: In total, 2 mL of blood was collected, with between two to five blood smears prepared, fixed with absolute methanol, and stained with a 10% Giemsa solution. Blood was stored at −20 ☌ for molecular analysis targeting the 18S rRNA gene. Fragments of the organs (liver, spleen, heart, and kidney) were separated and stained with hematoxylin-eosin. (3) Results: A total of 40 turtles were screened and hemogregarines were observed in 34 (85%) individuals (3 Podocnemis expansa and 31 Podocnemis unifilis). Haemogregarina embaubali and six new species of Haemogregarina were observed and formally described as follows: H. (4) Conclusions: This study contributes to the diversity and knowledge of Brazilian fresh-water turtle blood parasites, using integrative approaches for diagnosing and characterizing hemoparasites, with the identification of six undescribed species. PCR amplification reactions were carried out in a final volume of 25 μL, containing 1 μL each of 10 pmol primers, 12.5 μL of Master Mix MyFi™ Mix Bioline ®, and 5 μL of extracted DNA, with nuclease-free water accounting for the remaining volume. PCR amplification was performed on a Peltier 200 Thermocycler (MJ Research, Watertown, MA) following the conditions of O’Dwyer et al. download full Version Big Fish Free Download PC Game Free Big Fish For Game Free download Game Big Fish Full Version Rip Compressed Games Big Fish for PC Game Ahsan Hussain.PCR products were subjected to gel electrophoresis at 80 V in a 1.5% agarose gel, stained with gel red, and observed using an ultraviolet transilluminator. At the present, more than 8888 free full games PC are loaded on the website and it has. Animals and Pets Anime Art Cars and Motor Vehicles Crafts and DIY Culture, Race, and Ethnicity Ethics and Philosophy Fashion Food and Drink History Hobbies Law Learning and Education Military Movies Music Place Podcasts and Streamers Politics Programming Reading, Writing, and Literature Religion and Spirituality Science Tabletop Games.

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